Protein Purification Reagents and Kits
Biotium c-Myc(MYC275), CF740 conjugate, 0.1mg/mL
The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Phospho-ULK1 (Ser556) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Human Phospho-ULK1 (Ser556) Detection Kit, 50,000 Assay Points
IBA LifeSciences Strep-TactinXT 4Flow high capacity resin; 20 ml
Strep-TactinXT 4Flow high capacity is a 4% agarose coupled with the streptavidin variant Strep-TactinXT and applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and high binding affinity, the resin is recommend for all protein classes, especially for low abundant proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow high capacity allows the application of denaturing agents such as urea. In comparison to Strep-TactinXT 4Flow, Strep-TactinXT 4Flow high capacity has a higher density of immobilized Strep-TactinXT, which leads to a 2-fold higher yield of purified protein. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography.
American Research Products Inc Mouse N myc interactor NMI E
Mouse N-myc-interactor (NMI) ELISA kit from CUSABIO Detection Range 15 6 pg/mL-1000 pg/mL Sensitivity 3 9 pg/mL Sample serum plasma tissue homogenates cell lysates Method Sandwich
ABclonal Technology Affinity Gel-conjugated Rabbit anti Myc-Tag mAb {Anti-Myc-Tag 亲和凝胶}
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ABclonal Technology Phospho-c-Myc-T58 Rabbit pAb
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Neuromics, Inc 10x Buffer BXT, Strep-Tacting XT Elution Buffer, 25 ml contains a Biotin concentration for competitve displacement of purified recombinant proteins.
Buffer BXT is a Strep-TactinXT Elution Buffer with Biotin. It contains a Biotin concentration for competitive displacement of purified recombinant Strep-tagII or Twin-Strep-tag fusion proteins from immobilized Strep-TactinXT.Buffer BXT is also recommended for efficient elution in combination with Strep-TactinXT and MagStrep "type3" XT beads (2-4090-002).
Medchemexpress LLC Myc protein, human (C-terminal 6xHis) | >95.0% | 100UG
Recombinant full-length human MYC (c-Myc) protein expressed in Escherichia coli and supplied as a lyophilized powder with a C-terminal 6xHis tag. The product is formulated in PBS with trehalose for stability, tested for purity and endotoxin, and supplied with storage and reconstitution guidance for research use.
- Species human.
- Expression system Escherichia coli.
- Tag C-terminal 6xHis.
- Purity greater than 95% (SDS-PAGE).
- Approximate molecular weight 58 kDa.
- Lyophilized formulation from PBS, pH 7.4, with 8% trehalose.
- Endotoxin level less than 1 EU/μg.
- Store at -20°C; stable after reconstitution at 4°C for 1 week or -20°C for long term.
Revvity Health Sciences Inc HTRF Strep-Tactin Tb-Conjugate, 5,000 Assay Points
HTRF Strep-Tactin Tb-Conjugate, 5,000 Assay Points
Norgen Biotek Corp ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is also highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl2, NaCl, KCl, CaCl2, LiCl and CsCl. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit convenient for preparing proteins before prior to downstream applications such as SDS-PAGE, X-ray crystallography, mass spectroscopy and other applications.The kit is also designed for the rapid removal of endotoxins from up to 200 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved. This kit efficiently reduces endotoxin levels to ≤ 0.01 EU/mg of protein. Purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms.
IBA LifeSciences Strep-TactinXT 4Flow cartridge; 5 ml
Strep-TactinXT 4Flow cartridges contain a 4% agarose coupled with the streptavidin variant Strep-TactinXT and are applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins with HPLC/FPLC devices like Äkta systems (Cytiva). Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow allows the application of denaturing agents such as urea.
Biotium c-Myc(9E10.3), CF740 conjugate, 0.1mg/mL
The epitope of this antibody spans the Myc tag epitope, aa 410-419 (EQKLISEEDL), which is a specific portion of an alpha helical region of human c-myc protein. Because the epitope is located at the dimerization site of the protein, the antibody is not sensitive for detecting endogenous c-myc, making it suitable for detecting Myc tagged proteins or myc overexpression. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting lo
IBA LifeSciences Strep-Tactin TACS Agarose
Strep-Tactin TACS Agarose contains agarose macrobeads coated with the streptavidin variant Strep-Tactin. A special crosslinking permits the use for cell isolation and depletion approaches. It was developed for column-based Traceless Affinty Cell Selection (Fab-TACS/Nano-TACS). In this approach, Fab fragments fused to a Twin-Strep-tag (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin on the agarose matrix. After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin Agarose by biotin addition. In this way, the eluted cells are ready for further downstream applications. The same principle is applicable for exosome isolation (Fab-TACS for exosomes). Alternatively to Fab-Streps/Nano-Streps, it is possible to bind Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding) to this resin. This makes Strep-Tactin TACS Agarose also suitable for negative cell selection or depletion approaches.
IBA LifeSciences Strep-TactinXT 4Flow Starter Kit
Strep-TactinXT 4Flow Starter Kit contains all essential reagents required for purification and detection of Strep-tagII and Twin-Strep-tag fusion proteins. It provides a Strep-TactinXT 4Flow column, Buffer W, Buffer BXT, and Buffer XT-R for purification as well as Strep-Tactin HRP for subsequent western blot detection. Strep-TactinXT 4Flow columns contain a 4% agarose coupled with the streptavidin variant Strep-TactinXT. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow allows the application of denaturing agents such as urea.